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. 2018 Apr 18;19(4):1233. doi: 10.3390/ijms19041233

Figure 4.

Figure 4

Testosterone-dependent miRNAs exert a pro-viability function by inhibiting pro-apoptotic factors. (A) Potential binding sites predicted by TargetScan [40] and RNAhybrid [41] for miR-26a-5p and let-7g-5p in the 3′-UTR of PTEN and the 5′-UTR of PMAIP1, respectively, and the mutant PTEN 3′-UTR and PMAIP1 5′-UTR used in our study. A luciferase reporter assay was performed by co-transfecting luciferase reporter containing the 3′-UTR of PTEN and 5′-UTR of PMAIP1 (wild-type (Wt) or mutant (Mut)) with the mimic or control of miR-26a-5p and let-7g-5p into PK15 cells. The red underlined bases highlighted the miRNA seed sequences and their corresponding target sites in the mRNA UTR sequences. Luciferase activity was determined 48 h after transfection for (B) miR-26a-5p and (C) let-7g-5p. MC, mimics control. Three independent experiments were performed in triplicate and all data are expressed as means ± SD. ** p < 0.01.