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. 2018 Mar 24;19(4):975. doi: 10.3390/ijms19040975

Figure 3.

Figure 3

FBXO28 does not regulate β-cell function in human islets. Freshly isolated human islets of nondiabetic organ donors were infected with LacZ control or FBXO28 adenoviruses (A–F) or with Ad-GFP-shScr control or Ad-GFP-shFBXO28 (G–L) for 2 days. (A,G) Insulin content analyzed after GSIS and normalized to whole islet protein. (B,H) Insulin secretion during 1 h-incubation with 2.8 mM (basal) and 16.7 mM glucose (stimulated), normalized to insulin content. (C,I) The insulin stimulatory index denotes the ratio of secreted insulin during 1 h-incubation with 16.7 mM and 2.8 mM glucose. (D,J) RT-PCR for NEUROD1, MAFA, PDX1, INS, GCK, NKX6.1, NKX2.2 and SlC2A2 normalized to Cyclophilin. FBXO28 mRNA (E,K) and protein (F,L) expression in human islets confirm successful FBXO28 overexpression (E,F) and downregulation (K,L). Pooled data are from at least four independent experiments from at least four different human islet donors. Data show means ± SEM. * p < 0.05 compared to Ad-LacZ (D,E) or Ad-shScr (J,K).