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. 2018 Mar 24;19(4):975. doi: 10.3390/ijms19040975

Figure 4.

Figure 4

FBXO28 does not improve β-cell function in T2D human islets and FBXO28 mRNA expression is unchanged in T2D islets. (A–D) Similar to non-diabetic human islets in Figure 3, freshly isolated human islets from patients with type 2 diabetes (T2D) were infected with LacZ control or FBXO28 adenoviruses for 1 day. (A) Insulin content analyzed after GSIS and normalized to whole islet protein; (B) Insulin secretion during 1 h-incubation with 2.8 mM (basal) and 16.7 mM glucose (stimulated), normalized to insulin content; (C) The insulin stimulatory index denotes the ratio of secreted insulin during 1 h-incubation with 16.7 mM and 2.8 mM glucose; (D) FBXO28 mRNA expression in human T2D islets; (E) RT-PCR for FBXO28 mRNA expression in human islets isolated from nondiabetic (n = 24) or individuals with T2D (n = 7), normalized to Cyclophilin. (A–D) Pooled data are from five independent experiments from five different human islet donors with confirmed T2D. Data show means ± SEM. * p < 0.05 compared to Ad-LacZ.