Figure 3.

Trans-complementation and direct repeat deletion assay. The frequency of GFP reconstitution is shown on the left axis and represented by gray bars, whereas the titers are shown as a log scale on the right axis and represented by white bars. The error bars represent the SEM of at least two independent experiments. Squares represent the polymerase domain, and circles represent the RNase H domain of RT. White squares and circles represent wild type; black squares and circles represent replication-defective mutants. Mutants separated by a “/” represent trans-complementation; mutants with a “+” represent mutations contained within the same RT.