Figure 3.
MiR-146a deficiency promotes bacterial clearance both in vitro and in vivo. (A) WT and miR-146a KO mice were infected with L. monocytogenes 10403S at a dose of 1 × 106 CFU (i.p.). Mice were sacrificed on day 1 post-infection, and the bacterial load in the liver was determined (n = 12); (B) bacterial load in the spleen on day 1 (n = 12); (C) bacterial load in the liver on day 3 (n = 12); (D) bacterial load in the spleen on day 3 (n = 12); (E) WT and miR-146a KO BMDMs were incubated with L. monocytogenes 10403S at an MOI of 10 for 6 h, and the number of live bacteria in macrophages was determined; (F) lactate dehydrogenase (LDH) release in WT and miR-146a KO BMDMs. A total of 5 × 105 BMDMs were infected for 6 h at an MOI of 10. Data are shown as the mean ± s.e.m. of three independent experiments. * p < 0.05; ** p < 0.01; *** p < 0.001.