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. 2018 Mar 23;19(4):957. doi: 10.3390/ijms19040957

Figure 1.

Figure 1

AngII increases the Etd+ uptake in MES-13 cells. (A) Phase contrast and fluorescence images show MES-13 cells exposed to ethidium (5 μM Etd+) for 13 min under AngII stimulation (10−7 M) at different periods of time (0, 24, 48 and 72 h). Scale bar = 50 μm; (B) Representative time lapse of Etd+ uptake in mesangial cells recorded every 30 s, each plotted point corresponds to the mean of 20 cells, mean value ± SE. Dye uptake curve after application of AngII at different times (0, 24, 48 and 72 h); (C) Etd+ uptake rate of mesangial cells under basal conditions (0 h) or after treatment with AngII for different times (24, 48, and 72 h), with or without FBS (Fetal bovine serum) and Losartan (20 μM). Inset shows propidium iodide uptake rate of mesangial cells under basal conditions or after treatment with AngII for 72 h. Each bar represents the mean value ± SE of ≥3 independent experiments. Statistical significance *** p < 0.001 vs. 0 h; ### p < 0.001 vs. AngII 72 h. Treatments with AngII for 72 h are denoted below each bar with a plus sign (+). Losartan was co-treated with AngII for 72 h.