Figure 4.

ROCK, Cx43 HC, Panx1 Ch and P2X₇R blockers reduce the AngII-induced cell membrane permeabilization in MES-13 cells. Quantification of the Etd⁺ uptake rate in MES-13 cells under control conditions, exposed to AngII (10⁻⁷ M) for 72 h with or without different blockers: Fasudil (15 μM) and Y-27632 (15 μM), ROCK Inhibitors; apyrase (2 units/mL), ATP hydrolase; probenecid (PBC, 500 µM), Panx1 Ch blocker. Lanthanum ion (La³⁺, 200 μM), Cx43 HC and P2X₇R blocker; the mimetic peptide Gap27 (100 µM), a selective Cx43 HCblocker; carbenoxolone (CBX 10 μM), a Panx1 Ch blocker; A740003 (100 μM), a selective P2X₇R blocker. Each bar represents the mean value ± SE of ≥3 independent experiments. Statistical significance *** p < 0.001 vs. control; ^### p < 0.001 vs. AngII. Gap27, apyrase, PBC, Fasudil and Y-27632, were added during the last 24 h of treatment with AngII. Acutely were added Gap27 (added 15 min before each Etd⁺ uptake measurement), La³⁺, CBX, A740003, fasudil and Y27632 (each was added 10 min after Etd⁺ uptake). Each treatment with AngII for 72 h is denoted below each bar with a plus sign (+).