Figure 9.

Blockade of ROCK, Cx HCs or P2X₇Rs decreases the amount of TBARS, TNF-α and IL-1β in AngII-treated MES-13 cells. Graphs showing amount of (A) TBARS, (B) IL-1β and (C) TNF-α in the culture medium of MES-13 cells under control conditions or after 72 h exposure to AngII (10⁻⁷ M) in the presence of different blockers: Gap27 (100 µM), a selective Cx43 HC blocker; probenecid (PBC, 500 µM), Panx1 Ch blocker; A740003 (100 μM), a selective P2X₇R blocker or Fasudil (15 μM), ROCK inhibitor applied 24 h before harvesting the medium samples. Each bar represents the mean value ± SE of 4 independent experiments. Statistical significance, *** p < 0.001 vs. Ctrl; ^### p < 0.001 vs. AngII; ^&&& p < 0.001 vs. AngII + PBC.