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. 2018 Apr 4;19(4):1075. doi: 10.3390/ijms19041075
AF594-IB4 Alexa Fluor 594-labeled BS-I-B4 isolectin
α-GalT α-1,3-galactosyltransferase
CAG Chicken β-actin-based promoter
CRISPR/Cas9 Clustered regularly interspaced short palindromic repeats (CRISPR)/CRISPR-associated protein-9 nuclease
D-PBS Dulbecco’s modified phosphate-buffered saline without Ca2+ and Mg2+, pH 7.4
ds Double-stranded
DSBs Double-stranded breaks
GFP Green fluorescent protein
EndoGalC Endo-β-galactosidase C
ES Embryonic stem
FACS Fluorescence-activated cell sorting
FBS Fetal bovine serum
Dgcr2 DiGeorge syndrome critical region gene 2
DMEM Dulbecco’s modified Eagle’s medium
FBS Fetal bovine serum
GAAT1 α-1,3-galactosyltransferase
gRNA Guide RNA
GFP Green fluorescent protein
hCas9 Humanized Cas9
HR Homologous recombination
IB4SAP Saporin toxin-labeled BS-I-B4 isolectin
iPS Induced pluripotent stem
indels Indel mutations
KO Knockout
LDLR Low density lipoprotein receptor
NHEJ Nonhomologous-end-joining
oligos Oligonucleotides
pac Puromycin N-acetyltransferase gene
PAM Protospacer adjacent motif
PAPC Porcine adipocyte precursor cells
PGK Phosphoglycerate kinase
PEF Porcine embryonic fibroblasts
PCR Polymerase chain reaction
PFA Paraformaldehyde
PTEN Phosphatase and tensin homolog from chromosome 10
RNP Ribonucleoprotein complexes
SAP Saporin
SCNT Somatic cell nuclear transfer
T7E1 T7 Endonuclease assay
TALEN Transcription activator-like effector nucleases
TGFβRI Transforming growth factor-β receptor type 1 gene
VPA Valproic acid
ZFN Zinc-finger nuclease