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. 2018 Apr 18;19(4):1229. doi: 10.3390/ijms19041229

Figure 3.

Figure 3

Detection of the beaver pep/PAG-L produced in vitro by beaver placental explants. (A) secretory and cellular proteins (10 µg/sample) separated by SDS-PAGE and stained by Coomassie Brillant Blue. (B) Western analysis with anti-porcine PAG-P polyclonals (1:300). Abbreviations: #—animal code; Cn—negative control (endometrial proteins of cycling pigs); Cp—positive control (porcine placental proteins); F—female fetus; M—male fetus; fractions (1–5) of secretory proteins harvested up to 209 h during in vitro cultures; 0—pre-culture; media collected after: 1—6 h, 2—18 h, 3—30 h, 4—42 h, 5—54 h of culture; H—cellular proteins; M—molecular marker. Note: arrowshead indicate dominant isoforms of the pep/PAG-L.