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. 2018 Apr 18;19(4):1229. doi: 10.3390/ijms19041229

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© 2018 by the authors.

Licensee MDPI, Basel, Switzerland. This article is an open access article distributed under the terms and conditions of the Creative Commons Attribution (CC BY) license (http://creativecommons.org/licenses/by/4.0/).

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Detection of the beaver pep/PAG-L within labyrinth sections. Immuno-localization of pep/PAG-L signals within the labyrinth sections, identified by htdF-IHC with anti-pPAG-P (A,B) or anti-Rec pPAG2 polyclonals (C), visualized by goat anti-rabbit IgG-conjugated with Alexa 488 dye (A488; green) among all placental cells with nuclei stained by propidium iodine (red). (D) Negative control with omitted primary antibodies and nuclei stained by propidium iodine. Abbreviations: TR—trophectoderm; LZ—labyrinth zone.