Table 3.
Specific primers applied for the beaver placental AP cDNA amplification.
| Ordinal No. of Primer Pairs | Primers Name | Sequence (5′–3′) | Position a | Amplicon Length (bp) a |
|---|---|---|---|---|
| 1 | ATG_F | ATGAAGTGGATAGTGGTGGCC | 1–21 | ~1110 |
| 9_R | GAAGACATCWCCMAGGATCCAA | 1089–1110 | ||
| 2 | 2_F | TTCCTGAAGAMSCACAA | 124–140 | ~436 |
| 5_R | TAGGCCAGSCCCAKGATGCCATC | 538–560 | ||
| 3 | 3_F | GCTCCTCCAACCTGTGGGT | 287–305 | ~568 |
| 7_R | CAGAGAGGTGCCKGTGTCMACAA | 833–855 | ||
| 4 | 5_F | GATGGCATCMTGGGSCTGGCCTA | 538–560 | ~572 |
| 9_R | GAAGACATCWCCMAGGATCCAA | 1089–1110 | ||
| 5 | 7_F | TTGTKGACACMGGCACCTCTCTG | 833–855 | ~424 |
| 3′utr_R | CCAGAGAAGAGGCACAGATAGA | 1236–1257 |
a Position and amplicon length was estimated due to the beaver placental AP sequence identified using RNA-seq.