Figure 2.

Effects of hinokitiol on phosphorylation of p53 and Bax in A549 cells. A549 adenocarcinoma (5 × 10⁴ cells/well) cells were treated with different concentrations (1–5 µM) of hinokitiol for 30 min. The phosphorylated p53 and Bax proteins in the cell lysate were assayed by Western blotting. Effects of hinokitiol on phosphorylation of p53 (A) and Bax (B) in A549 adenocarcinoma cells. α-tubulin was used as an internal control. The figures are representative examples of three independent experiments. Data are shown as the mean ± standard errors of the means (SEM) of three independent experiments. * p < 0.05, and ** p < 0.01 compared with untreated A549 cells.