Luciferase response (LUC, bars) and cell viability (circles) of MELN (A), ZELHα (B), ZELHβ1 (C), ZELHβ2 (D), ZELH (E) and ZFL (F) cells after exposure to TCS (0.03 to 30 µM) for 24 h (MELN) or 72 h (ZELH-zfERs), in presence (dark symbols) or absence (light symbols) of E2. MELN, ZELHβ1 and ZELHβ2 cells were co-exposed to TCS + E2 0.1 nM, and ZELHα, ZELH and ZFL cells to TCS + E2 1 nM. Data represent the mean (+/− SD) of a minimum of 3 independent experiments using technical triplicates. Data were normalized to E2 positive control for luciferase activity. SC: solvent control ¤: response significantly different from control cell viability *: response significantly different from control luciferase activity (Mann-Whitney, p < 0.05).