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. 2018 Mar 29;19(4):1030. doi: 10.3390/ijms19041030

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© 2018 by the authors.

Licensee MDPI, Basel, Switzerland. This article is an open access article distributed under the terms and conditions of the Creative Commons Attribution (CC BY) license (http://creativecommons.org/licenses/by/4.0/).

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Resveratrol reverses the retinoic acid sensitivity of THJ-11T cells. (A) H/E staining (×40); (B) deoxynucleotidyl transferase-mediated dUTP-biotin nick and labeling assay (TUNEL) for apoptotic cell labeling (Green in color; ×40); (C) MTT cell proliferation assay; (D) viable cell counting; (E) immunocytochemical staining of thyroglobulin (Tg) and E-cadherin (×40). Control, cultured in 0.2% dimethylsulfoxide (DMSO)-containing medium; RA, retinoic acid treatment; Res, resveratrol treatment; Resveratrol plus RA, treated with a combination of 10 µM retinoic acid and 100 µM resveratrol for 48 h; Resveratrol-alone, 100 µM resveratrol; *, with statistical significance (p < 0.05); the error bars, the mean ± standard deviation. Arrows indicate the ports with higher magnification (×80) in the insets.

Resveratrol reverses the retinoic acid sensitivity of THJ-11T cells. (A) H/E staining (×40); (B) deoxynucleotidyl transferase-mediated dUTP-biotin nick and labeling assay (TUNEL) for apoptotic cell labeling (Green in color; ×40); (C) MTT cell proliferation assay; (D) viable cell counting; (E) immunocytochemical staining of thyroglobulin (Tg) and E-cadherin (×40). Control, cultured in 0.2% dimethylsulfoxide (DMSO)-containing medium; RA, retinoic acid treatment; Res, resveratrol treatment; Resveratrol plus RA, treated with a combination of 10 µM retinoic acid and 100 µM resveratrol for 48 h; Resveratrol-alone, 100 µM resveratrol; *, with statistical significance (p < 0.05); the error bars, the mean ± standard deviation. Arrows indicate the ports with higher magnification (×80) in the insets.