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. 2018 Mar 29;19(4):1030. doi: 10.3390/ijms19041030

Figure 5.

Figure 5

Immunocytochemical and Western blotting demonstration of resveratrol-induced alterations of RAR-β and PPAR-β/δ expression and their intracellular distribution patterns in THJ-11T cells. (A) Immunocytochemical staining (×40); (B) ImageJ-based quantification of nuclear labeling of RAR-β and PPAR-β/δ; (C) Levels of RAR-β and PPAR-β/δ determined by Western blotting. Control, 0.2% DMSO-treated cells; Resveratrol, 48 h 100 µM resveratrol treatment. Arrows indicate the portions in higher magnification in the insets (×80). Ratio, ratio between the levels of the target molecules and that of β-ACTIN; *, with statistical significance (p < 0.05); NS, no statistical significance (p > 0.05); the error bars, the mean ± standard deviation. Gray values, nuclear RAR-β and PPAR-β/δ immunocytochemical staining densities.