Figure 4.
The MTT assay and flow cytometry analysis to compare cellular toxicity and apoptosis induction of varying treatments on HeLa and HT-3 cells. (a) Cell viability of HeLa and HT-3 cell lines following incubation with liposomal encapsulated ATO (ATO lipo) and free ATO for 24, 48 and 72 h; (b) Flow cytometry analysis of apoptotic populations that were treated with complete media as control, empty liposomes, liposomal 5 µM ATO, or 5 µM free ATO on HeLa and HT-3 cell lines following treatment for 24 and 48 h; (c) Calculated IC50 values for ATO lipo and free ATO treated HeLa and HT-3 cells after 24, 48, and 72 h. Data are presented as mean ± SD of three replicate experiments.