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. 2018 Mar 25;19(4):977. doi: 10.3390/ijms19040977

Figure 2.

Figure 2

Coupling percentage and Gj of AF-linked mutants. (A) Dual whole-cell patch clamp technique was used to measure junctional current (Ij) from N2A cell pairs expressing untagged Cx40, K107R, L223M, Q236H, or I257L at 20 mV Vj; (B) bar graph summarizes the coupling percentages of cell pairs expressing the AF-linked Cx40 mutants. No statistical difference was observed between each of the mutants and the wildtype Cx40. The number of transfections is indicated on each bar; (C) bar graph illustrates the coupling conductance (Gj) of coupled cell pairs expressing Cx40, K107R, L223M, Q236H, or I257L. Cell pairs expressing Q236H showed a significantly lower Gj than those of wildtype Cx40 (* p < 0.05). The number of cell pairs is indicated on each bar.