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. 2018 Mar 25;19(4):977. doi: 10.3390/ijms19040977

Figure 7.

Figure 7

Propidium iodide uptake of AF-linked Cx40 mutants. (A) HeLa cells transfected with Cx40 mutants, empty vector GFP, or Cx40 are shown: column 1 (under DIC), column 2 (GFP fluorescence to show successful expression of respective vector), column 3 (propidium iodide [PI] uptake in red), column 4 (an overlay of images of column2 and 3). Only cells expressing L221I showed PI uptake. The scale bar = 50 μm; (B) bar graph summarizes PI uptake percentage of isolated individual cells expressing Cx40 mutants, Cx40, or GFP. PI uptake percentage for each of the AF-linked mutants was not statistically different from that of wildtype Cx40 or the empty vector (GFP), except L221I (*** p < 0.001). The number transfection is indicated with observations of over 60 isolated cells for each transfection.