Table 1.
Boltzmann fitting parameters for Vj gating of AF-linked mutants.
| Cells Expressing | Vj Polarity | Gmin | V0 (mV) | A |
|---|---|---|---|---|
| Cx40 (n = 7) |
+ | 0.25 ± 0.02 | 40.2 ± 1.4 | 0.15 ± 0.05 |
| − | 0.27 ± 0.02 | 42.9 ± 1.4 | 0.19 ± 0.07 | |
| K107R (n = 5) |
+ | 0.21 ± 0.03 | 38.6 ± 1.6 | 0.15 ± 0.05 |
| − | 0.23 ± 0.03 | 41.1 ± 1.9 | 0.15 ± 0.05 | |
| L223M (n = 5) |
+ | 0.24 ± 0.02 | 40.2± 1.0 | 0.20 ± 0.10 |
| − | 0.31 ± 0.03 | 43.2 ± 1.8 | 0.17 ± 0.06 | |
| Q236H (n = 6) |
+ | 0.20 ± 0.02 | 33.3 ± 1.7 *,1 | 0.19 ± 0.04 |
| − | 0.24 ± 0.02 | 35.2 ± 2.0 * | 0.15 ± 0.04 | |
| I257L (n = 5) |
+ | 0.24 ± 0.03 | 40.9 ± 2.2 | 0.12 ± 0.03 |
| − | 0.24 ± 0.03 | 43.4 ± 2.2 | 0.17 ± 0.08 |
1 One-way ANOVA followed by Tukey post-hoc test was used to compare the Boltzmann fitting parameters of each mutant and wildtype Cx40 at the corresponding Vj polarity. GJ channels formed by the mutant Q236H showed a significantly lower V0 for both Vj polarities than those of wildtype Cx40 (* p < 0.05).