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Immortalised NF and CWF cell lines express hTERT and template component (hTR) as demonstrated by RT-PCR (A) and have active telomerase as demonstrated by the appearance of a 6 bp ladder (B). CWF immortalisation and maintenance in culture (C) changes the appearance of the chronic cells from flattened and spread out to more bi-polar (and thereby more like the appearance of the NF cells). Scale bar = 20 µm.
