Table 4.

Pathways/processes that were significantly over represented after immortalisation, that is were up-regulated in hTERT cells (i.e., only those associated with cell proliferation [Cell Cycle, Mitosis and DNA Replication] or telomere maintenance were altered).

Pathway	Genes
DNA replication	TOP2A, MCM3, RRM2, MCM6, NASP, CDC6, RFC3, CDK2, FEN1, CHEK1, POLE2, GINS1, TERT, MCM7, NF2, CIZ1, GMNN, DTL, PDGFC, MCM4
DNA strand elongation involved in DNA replication	MCM3, MCM6, RFC3, FEN1, GINS1, MCM7, MCM4
cell cycle	TOP2A, MCM3, RANBP2, RRM2, MCM6, NASP, TUBB3, GAS7, CCNA2, TFDP2, FGFR2, RARA, MAPRE3, CDC6, ZWINT, RFC3, CKS2, CDK2, RASSF1, FEN1, BARD1, CHEK1, POLE2, GINS1, TUBB2C, HMG20B, SMAD6, TGFB2, TPX2, MCM7, KIF2C, TUBB, CDKN2C, ARAP1, CDKN1C, CKAP2, GMNN, CEP55, DTL, MCM4, RACGAP1
cell cycle phase	POLD2, TOP2A, PSMD3, MCM3, RRM2, CHMP1A, TUBB3, DNM2, RANBP1, UBE2S, RFC5, CDK1, CAV2, MAD2L1, CCNA2, BUB1B, DLGAP5, CDC6, ZWINT, TRIP13, AURKA, CKS2, DBF4, CDK2, PSMB9, GTSE1, CDC7, KIF23, FEN1, CCNF, CENPA, GINS1, AKT1, DGKZ, TUBB2C, TUBB, ID4, BUB1, CDKN3, HGF, TPX2, CDKN2D, SSNA1, MCM7, KIF2C, KPNA2, RANGAP1, TFDP1, NEK1, TCF3, MDM2, PRC1, NUSAP1, GMNN, CEP55, PBK, CDKN1C, CENPN, NCAPG2, TBRG4, CCDC99, MCM4
organelle fission	CHMP1A, TUBB3, RANBP1, CDK1, CAV2, MAD2L1, CCNA2, BUB1B, DLGAP5, CDC6, ZWINT, AURKA, CDK2, KIF23, CCNF, CENPA, TUBB, BUB1, HGF, TPX2, KIF2C, RANGAP1, NEK1, NUSAP1, CEP55, PBK, CENPN, NCAPG2, CCDC99
mitotic cell cycle	POLD2, TOP2A, PSMD3, MCM3, RRM2, CHMP1A, TUBB3, DNM2, RANBP1, UBE2S, RFC5, CDK1, CAV2, MAD2L1, CCNA2, BUB1B, DLGAP5, CDC6, ZWINT, AURKA, DBF4, CDK2, PSMB9, GTSE1, CDC7, KIF23, FEN1, CCNF, CENPA, GINS1, AKT1, DGKZ, TUBB2C, TUBB, ID4, BUB1, CDKN3, HGF, TPX2, CDKN2D, SSNA1, MCM7, KIF2C, KPNA2, RANGAP1, TFDP1, NEK1, TCF3, MDM2, PRC1, NUSAP1, GMNN, CEP55, PBK, CDKN1C, CENPN, NCAPG2, TBRG4, CCDC99, MCM4
cell division	PPP1CA, CHMP1A, UBE2S, CDK1, MAD2L1, CCNA2, FGFR2, BUB1B, CDC6, ZWINT, AURKA, CKS2, CDK2, CDC7, KIF23, CCNF, FGF1, FGF5, MDK, PTN, BUB1, TPX2, KIF2C, NEK1, PRC1, NUSAP1, CEP55, PDGFC, PDGFD, NCAPG2, CCDC99, RACGAP1
mitosis	CHMP1A, TUBB3, RANBP1, CDK1, CAV2, MAD2L1, CCNA2, BUB1B, DLGAP5, CDC6, ZWINT, AURKA, CDK2, KIF23, CCNF, CENPA, TUBB, BUB1, HGF, TPX2, KIF2C, RANGAP1, NEK1, NUSAP1, CEP55, PBK, CENPN, NCAPG2, CCDC99
M phase	TOP2A, CHMP1A, TUBB3, RANBP1, UBE2S, CDK1, CAV2, MAD2L1, CCNA2, BUB1B, DLGAP5, CDC6, ZWINT, TRIP13, AURKA, CKS2, CDK2, KIF23, CCNF, CENPA, TUBB, BUB1, HGF, TPX2, KIF2C, KPNA2, RANGAP1, NEK1, PRC1, NUSAP1, CEP55, PBK, CENPN, NCAPG2, CCDC99
DNA replication	RFC2, POLD2, PCNA, TOP2A, RRM1, RPA1, MCM3, MCM5, RRM2, MCM6, NASP, MCM2, TK1, TYMS, RNASEH2A, RFC5, CDC6, RFC4, RFC3, DBF4, POLA2, FEN1, CHAF1B, POLA1, RAD51, CCNE2, CHEK1, PDGFA, CIZ1, GINS1, TERT, CENPF, MCM7, DUT, CDT1, NF2, ORC5, MCM4, CDC34, GMNN, DTL, RMI1, TIPIN, MCM10, GINS2
cell cycle checkpoint	RFC2, TOP2A, BUB3, RPA1, MCM3, MCM5, MCM6, BIRC5, MCM2, CCNB2, CDC20, UBE2C, RFC5, CDK1, MAD2L1, CCNA2, FANCG, BUB1B, CDC6, RFC4, ZWINT, RFC3, DBF4, PSMB9, GTSE1, TTK, POLA1, CCNE2, CHEK1, CENPF, MCM7, BUB1, CDT1, ORC5, MCM4, CCNB1, ZWILCH, DTL, TIPIN, MCM10
cell division	PPP1CA, BUB3, NCAPD2, CDC25B, PSRC1, BIRC5, CCNB2, CDC20, UBE2C, CDK1, MAD2L1, CCNA2, FGFR2, BUB1B, CDC6, ZWINT, AURKA, NDC80, CKS2, SMC2, KIF11, NEK2, KIF23, CCNF, CCNE2, RAB35, PDGFA, DIAPH2, CENPF, MAEA, FGF5, MDK, KIF2C, AURKB, PTN, BUB1, TGFB2, TPX2, OIP5, CCNB1, PRC1, NUSAP1, ZWILCH, CEP55, FBXO5, TIPIN, NCAPG2, ERCC6L, ASPM, CDCA3, CDCA8, RACGAP1
S phase of mitotic cell cycle	RFC2, POLD2, PCNA, RPA1, MCM3, MCM5, MCM6, MCM2, BCL6, RFC5, CDC6, RFC4, RFC3, PSMB9, POLA2, FEN1, POLA1, GINS1, MCM7, CDT1, ORC5, MCM4, GINS2
telomere maintenance via recombination	RFC2, POLD2, PCNA, RPA1, RFC5, RFC4, RFC3, POLA2, FEN1, POLA1
telomere maintenance via semi-conservative replication	RFC2, POLD2, PCNA, RPA1, RFC5, RFC4, RFC3, POLA2, FEN1, POLA1
M/G1 transition of mitotic cell cycle	RPA1, MCM3, MCM5, MCM6, MCM2, CDC6, DBF4, PSMB9, POLA2, POLA1, MCM7, CDT1, ORC5, MCM4, GMNN, MCM10