Figure 1.

Liu Jun Zi Tang attenuated cisplatin-induced thermal hyperalgesia in mice. Eight-week-old Balb/c mice were housed in a cage and habituated for two weeks to avoid stress-induced analgesia. Cisplatin was freshly prepared in normal saline. Mice were treated with daily intraperitoneal injections for 5 days, followed by 9 days of rest, for two cycles. Liu Jun Zi Tang (LJZT) was administered 1 h prior to cisplatin administration. After the treatments, a tail-flick assay was performed to monitor tolerance time in response to heat. Data points represented the mean tolerance time ± standard deviation. Differences among multiple groups were evaluated using one-way analysis of variance. Differences between means were calculated using the Student-Newman-Keuls method and were considered significant at p < 0.05. * p < 0.05, compared with controls (n = 6–8).