Figure 3.

Effects of Liu Jun Zi Tang on cisplatin-induced cytosolic reactive oxygen species formation in human neuroblastoma (SH-SY5Y) cells. Cisplatin was administered for another 24 h with or without pretreatment with LJZT for 1 h. Cells were then stained with (A) di-hydroxyethidium (DHE) and (B) CO₃-H₂DCFDA and subjected to image acquisition and quantitation. At least 10 frames were randomly acquired for each group. Fluorescence intensity is expressed as a percentage of controls. Data are presented as the mean ± standard deviation. Differences among multiple groups were evaluated using one-way analysis of variance and those between means were calculated using the Student-Newman-Keuls method with significance set at p < 0.05. * p < 0.05, compared with controls (n = 3–6). # p < 0.05, compared with the cisplatin-only treatment group (n = 3–6). The bar represents 50 µm. These data represent one out of three independent experiments that provided similar results.