Table 5.
The validation results obtained between the correlation of RNA-seq and qPCR data for differentially expressed genes.
| Gene | Accession Number | Hampshire | Pietrain | R | ||
|---|---|---|---|---|---|---|
| FC RNA-seq |
FC RQ |
FC RNA-seq |
FC RQ |
|||
| MGP | ENSSSCG00000000606 | 2.45 | 2.07 | 1.63 | 1.80 | 0.928 *** |
| BBS2 | ENSSSCG00000025417 | −2.25 | −1.76 | −1.53 | −1.03 | 0.646 *** |
| PCOLCE | ENSSSCG00000027466 | 3.35 | 2.79 | 1.57 | 1.10 | 0.886 *** |
| COL6A1 | ENSSSCG00000022506 | 2.20 | 1.40 | 1.97 | 1.70 | 0.643 ** |
| TBX2 | ENSSSCG00000027060 | 2.05 | 1.91 | 2.21 | 1.09 | 0.360 ns |
| XPO1 | ENSSSCG00000028228 | −1.71 | −0.62 | −1.50 | −1.80 | 0.280 ns |
R—correlation coefficient; FC—fold change values between groups with high and low meat content in carcasses in each breed; RQ—relative quantity, Pearson correlation coefficient with p-value (***—p < 0.000; **—p < 0.01; ns—not significant).