Figure 2.
The height of F-actin-rich domains of the podosome-related structures of osteoclasts. Osteoclasts differentiated from RAW 264.7 cells on glass were stained with FITC (fluorescein isothiocyanate)-phalloidin for N-structured illumination microscopy (SIM) imaging. Z-stacks of the entire FITC-positive volume were acquired at 0.3 µm intervals. The images are representatives of cross sections of the podosome cluster, the podosome ring, the podosome belt, and the zipper-like structure.