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Western blotting was used to detect the expression of apoptosis-related proteins in HepaRG cells after treatment with various concentrations of rhein for 24 h. The β-actin was used as a loading control. Quantity One software was used to quantify the protein-related bands. Results are the mean ± S.D. (n = 3). LSD t-test was carried out. * p < 0.05, significantly different compared with vehicle control.
