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. 2018 Apr 2;19(4):1062. doi: 10.3390/ijms19041062

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© 2018 by the authors.

Licensee MDPI, Basel, Switzerland. This article is an open access article distributed under the terms and conditions of the Creative Commons Attribution (CC BY) license (http://creativecommons.org/licenses/by/4.0/).

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Characterization of NtCCD8 genes in tobacco (Nicotiana tabacum). (A) Putative exon/intron structure of the NtCCD8A and NtCCD8B genes. (B) Alignment of the putative NtCCD8A and NtCCD8B amino acid sequences with known CCD8 proteins from other plant species. Nt, Nicotiana tabacum; At, Arabidopsis thaliana. Ph, Petunia hybrida; Sl, Solanum lycopersicum. (C) Phylogenetic tree obtained for known CCD1, CCD4, CCD7 and CCD8 amino acid sequences; Nt, Nicotiana tabacum; At, Arabidopsis thaliana; Ph, Petunia hybrida; Sl, Solanum lycopersicum. Os, Oryza sativa; Ps, Pisum sativum; Zm, Zea mays; Cs, Crocus sativus; Rd, Rosa damascena. Accession numbers for the sequences used are as follows: AtCCD1 (At3g63520), SlCCD1a (AAT68187), SlCCD1b (AAT68188), NtCCD1a (AIL30506), NtCCD1b (AKO22630), CsCCD4a (ACD62476), CsCCD4b (ACD62477), RdCCD4 (ABY60886), NtCCD4 (AEI61930), OsCCD7 (Q7XU29), ZmCCD7 (NP_001183928), AtCCD7 (AEC10494), PsCCD7 (ABD67496), PhCCD7 (FJ790878), SlCCD7 (ACY39883), OsCCD8a (AP003296), OsCCD8b (AP003376), AtCCD8 (AEE86121), PsCCD8 (AY557341), SlCCD8 (AEH96363), and PhCCD8 (AY743219).

Characterization of NtCCD8 genes in tobacco (Nicotiana tabacum). (A) Putative exon/intron structure of the NtCCD8A and NtCCD8B genes. (B) Alignment of the putative NtCCD8A and NtCCD8B amino acid sequences with known CCD8 proteins from other plant species. Nt, Nicotiana tabacum; At, Arabidopsis thaliana. Ph, Petunia hybrida; Sl, Solanum lycopersicum. (C) Phylogenetic tree obtained for known CCD1, CCD4, CCD7 and CCD8 amino acid sequences; Nt, Nicotiana tabacum; At, Arabidopsis thaliana; Ph, Petunia hybrida; Sl, Solanum lycopersicum. Os, Oryza sativa; Ps, Pisum sativum; Zm, Zea mays; Cs, Crocus sativus; Rd, Rosa damascena. Accession numbers for the sequences used are as follows: AtCCD1 (At3g63520), SlCCD1a (AAT68187), SlCCD1b (AAT68188), NtCCD1a (AIL30506), NtCCD1b (AKO22630), CsCCD4a (ACD62476), CsCCD4b (ACD62477), RdCCD4 (ABY60886), NtCCD4 (AEI61930), OsCCD7 (Q7XU29), ZmCCD7 (NP_001183928), AtCCD7 (AEC10494), PsCCD7 (ABD67496), PhCCD7 (FJ790878), SlCCD7 (ACY39883), OsCCD8a (AP003296), OsCCD8b (AP003376), AtCCD8 (AEE86121), PsCCD8 (AY557341), SlCCD8 (AEH96363), and PhCCD8 (AY743219).