Figure 3.

CRISPR/gRNA-mediated NtCCD8 genes disruption. (A) The common target site selected for targeted NtCCD8A and NtCCD8B mutation. The target region is indicated by the nucleotides in red followed by PAM (proto-spacer adjacent motif). (B) Sequences of the mutated sites in homozygous ntccd8 mutant plants. Deletions and insertions are indicated by dashes and red letters, and the numbers on the right side indicate the size of the deletions and insertions. (C) Schematic representation of the mutated position in ntccd8 mutant plants. The position of premature stop codons caused by deletions and insertions is indicated by a red asterisk, the deleted “GGG” and inserted “A” bases are shown in blue and red lettering, respectively, and premature stop codons are shown in bold.