Table 2.
Segregation patterns of CRISPR/Cas9-induced mutations in the NtCCD8 genes during the T0 to T1 generations.
| Line a | Target Genes | T0 | T1 | ||
|---|---|---|---|---|---|
| Zygosity b | Genotype c | Segregation Ratio | Cas9 d | ||
| T0-3 | NtCCD8A | Bi-allele | i1,d2 | 5i1:10i1d2:5d2 | 19+: 1− |
| NtCCD8B | Bi-allele | d1,d3 | 5d1:12d1d3:3d3 | ||
| T0-5 | NtCCD8A | Bi-allele | i1,d2 | 6i1:10i1d2:4d2 | 16+: 4− |
| NtCCD8B | Bi-allele | d1,d2 | 5d1:6d1d2:9d2 | ||
| T0-14 | NtCCD8A | Bi-allele | i1,d2 | 3i1:12i1d2:5d2 | 18+: 2− |
| NtCCD8B | Bi-allele | i1a,i1b | 20i1 | ||
a Line name is in the format of T0-# with indicating the T0 generation, # indicating the plant #. b The zygosity of bi-allele in T0 plants is putative. c The genotype of individual plants according to the sequencing results. d +, the number of Cas9 sequences that were detected; −, the number of Cas9 sequences that were not detected. Mutation types: d#x and i#x; d and i indicate nucleotide deletions and insertions, respectively. ‘#’ represents the number of nucleotide deletions or insertions. ‘x’ is used to show the different mutations at the same target site. For example, i1a and i1b represent the insertion of one different nucleotide at the same site.