Figure 3.

Gene expression levels of NADPH oxidase subunits and the level of 8-iso-prostaglandin F_2α in ApoE KO mice exposed to CS. The mRNA levels of (a) p22phox and (b) gp91phox in the aortic tissues were determined by quantitative RT-PCR analysis. Data are normalized by the abundance of β-actin mRNA. Quantitative data are expressed relative to the values for the control group; (c) 24-h urinary 8-iso-prostaglandin F_2α was measured using the enzyme immunoassay kit. Data are mean ± SEM of six or seven animals per group. * p < 0.05 versus the control group.