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. 2018 Apr 11;19(4):1155. doi: 10.3390/ijms19041155

Table 2.

Yield, acid number (n) and carboxylic group substitution degree of the carboxylated dextran derivatives 6 and 9 and conjugation degrees of TSPO ligand conjugates 7, 8, 10 and 11.

Compound Yield % pH Analysis UV Analysis 1H-NMR Analysis Elemental Analysis SEC Analysis
n E1% c λmax (nm) TSPO Ligand Conjugation Degree Carboxylic Group Substitution Degree Residual Free Carboxylic Groups TSPO Ligand Conjugation Degree TSPO Ligand Conjugation Degree
(w/w) a (mg/g) b (g/g) e (mol/mol) f (g/g) e (mol/mol) g
1 62.8 d 253
4 76.5 d 254
6 82 55.7 0.51
7 68 23.2 250 0.37 0.23 0.31 0.28
8 51 25.2 254 0.33 0.26 0.28 0.25
9 80 54.2 0.55
10 71 36.4 251 0.58 0.18 0.55 0.37
11 54 43.6 254 0.57 0.20 0.52 0.35

a Based on the percentage by weight of the polymer synthesized and the starting dextran; b calculated as mg of NaOH per g of polymer; c determined by UV analysis in H2O at wavelength 254 nm; d determined by UV analysis in MeOH:H2O, at a ratio of 2:1, at a wavelength of 254 nm; e calculated as g of TSPO ligand/g of polymer; f calculated as number of moles of carboxylic groups of C-Dex per mole of glucose; g calculated with Equation (1).