Table 2.
Compound | Yield % | pH Analysis | UV Analysis | 1H-NMR Analysis | Elemental Analysis | SEC Analysis | |||
---|---|---|---|---|---|---|---|---|---|
n | E1% c | λmax (nm) | TSPO Ligand Conjugation Degree | Carboxylic Group Substitution Degree | Residual Free Carboxylic Groups | TSPO Ligand Conjugation Degree | TSPO Ligand Conjugation Degree | ||
(w/w) a | (mg/g) b | (g/g) e | (mol/mol) f | (g/g) e | (mol/mol) g | ||||
1 | 62.8 d | 253 | |||||||
4 | 76.5 d | 254 | |||||||
6 | 82 | 55.7 | 0.51 | ||||||
7 | 68 | 23.2 | 250 | 0.37 | 0.23 | 0.31 | 0.28 | ||
8 | 51 | 25.2 | 254 | 0.33 | 0.26 | 0.28 | 0.25 | ||
9 | 80 | 54.2 | 0.55 | ||||||
10 | 71 | 36.4 | 251 | 0.58 | 0.18 | 0.55 | 0.37 | ||
11 | 54 | 43.6 | 254 | 0.57 | 0.20 | 0.52 | 0.35 |
a Based on the percentage by weight of the polymer synthesized and the starting dextran; b calculated as mg of NaOH per g of polymer; c determined by UV analysis in H2O at wavelength 254 nm; d determined by UV analysis in MeOH:H2O, at a ratio of 2:1, at a wavelength of 254 nm; e calculated as g of TSPO ligand/g of polymer; f calculated as number of moles of carboxylic groups of C-Dex per mole of glucose; g calculated with Equation (1).