Figure 3.

Targeted disruption of the Spag17 gene in the testis. (A) Schematic representation of the L1L2_Bact_P cassette inserted in Chromosome 3. (B) Schematic representation of the Cre-recombination for the deletion of exon 5. (C) Genotyping results. (D) mRNA expression of Spag17 was evaluated by RT-PCR in extracts of testis from wild-type and knockout mice. (E) Representative western blot showing the absence of SPAG17 protein in the testis from knockout mouse. WT, wild-type; HET, heterozygous; KO, homozygous knockout.