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Pcdp1 and IFT20 localization in the manchette is dependent on SPAG17. Germ cells from wild-type and knockout mice were collected from adult mice and stained for proteins associated to manchette structures. (A) Pcdp1 and (B) IFT20 failed to localize to the manchette in the elongating spermatids from Spag17 knockout mice, and their localization appears diffused in the cytoplasm. Scale bar = 10 µm.
