Figure 4.

Functional CFTR production in transfected CFBE cells. (a) CFTR channel encoded by pBQ-S/MAR increases chloride currents in CFTR deficient cells. The graph shows the average current/voltage relationships in the presence of a cAMP-containing cocktail (+cAMP) of pBQ-S/MAR transfected (pBQ-S/MAR n = 4) and un-transfected CFBE cells (CFBE, n = 5) or plus 10 μM CFTR_inh-172 (pBQ-S/MAR + Inh) (n = 6). Data are means ± SEM; * p < 0.05; ** p < 0.01; Student’s t-tests. (b) Representative western blots of proteins extracted from cells transfected with pBQ-S/MAR or pBQ6.2 grown for 14 days after transfection, and polarized for 9 more days. Controls: mock, CFBE treated with transfection reagent only; CFBE and 16HBE un-transfected cells. Band B, immature form of CFTR; band C, mature CFTR.