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. 2018 Mar 29;19(4):1035. doi: 10.3390/ijms19041035

Table 7.

Sequences of primer pairs and amplification conditions for RT-qPCR.

Item Function Primer Sequences Tm (C) Production Size
STAT3 RT-PCR F: 5-CACTTCTGCCAAGGGAGACT-3 59.5 261 bp
R: 5-CGCGTATGCCCAATCTTGAC-3
β-actin Internal control F: 5-CACCAACTGGGACGACAT-3 61 202 bp
R: 5-ATACAGGGACAGCACAGC-3
GAPDH Internal control F: 5-CCAACGTGTCTGTTGTGGAT-3 61 80 bp
R: 5-CTGCTTCACCACCTTCTTGA-3