Table 7.
Item | Function | Primer Sequences | Tm (◦C) | Production Size |
---|---|---|---|---|
STAT3 | RT-PCR | F: 5-CACTTCTGCCAAGGGAGACT-3 | 59.5 | 261 bp |
R: 5-CGCGTATGCCCAATCTTGAC-3 | ||||
β-actin | Internal control | F: 5-CACCAACTGGGACGACAT-3 | 61 | 202 bp |
R: 5-ATACAGGGACAGCACAGC-3 | ||||
GAPDH | Internal control | F: 5-CCAACGTGTCTGTTGTGGAT-3 | 61 | 80 bp |
R: 5-CTGCTTCACCACCTTCTTGA-3 |