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. 2018 Apr 4;19(4):1082. doi: 10.3390/ijms19041082

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© 2018 by the authors.

Licensee MDPI, Basel, Switzerland. This article is an open access article distributed under the terms and conditions of the Creative Commons Attribution (CC BY) license (http://creativecommons.org/licenses/by/4.0/).

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Loss of Squid causes severe changes in TBPH localization. (A,B) Compared to WT (A), loss of Squid changes TBPH protein (red) distribution between nucleus and cytoplasm in MT of Squid^- null mutants (B,C). Indeed, TBPH loses its co-localization with hsrω (green) and it is predominantly distributed in the cytoplasm (Red arrows). (C,D) Western blots confirming the data for MT cells (C) as well as BCs (D). (C’,D’) The intensity of Western blot signals was quantified using ImageJ. The experiment was performed considering five biological replicates. The error bars show the standard deviation. Unpaired Student’s t-test was performed to assay statistical significance; * 0.01 ≤ p-value ≤ 0.05; ** p-value < 0.01.