Figure 7.
gga-miR-451 maintains DF-1 cells at the G1 phase in MG-HS infection. DF-1 cells were transfected with miR-451-M, miR-451-NC, miR-451-Inh, or miR-451-Inh-NC and were incubated for 24 h. The cells then were infected with MG-HS strain. Four control groups, including miR-451-NC (MG+), miR-451-Inh-NC (MG+), miR-free (MG+), and blank (MG−), were used. At 24 h post-infection, the cell phase distribution was analyzed using a flow cytometer. The FL2-A channel (x axis) shows the DNA number. Three independent experiments, each with three replicates, were performed. The data are presented as the means ± SDs. One-way ANOVA was used to analyze significant differences (Different lowercase letters represent p < 0.01 in G1 and S + G2 phase, respectively).