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. 2018 Mar 21;19(4):932. doi: 10.3390/ijms19040932

Figure 2.

Figure 2

Response to exogenous 17β-estradiol treatment in 6-month-old juvenile female trout. Fish were fed with E2 containing pellets for five days and pellets prepared with only vehicle (ethanol) were used as control diets. (A). Absolute quantification of vitellogenin (VTG) mRNA in the liver and E2 levels in serum of the control (C) and E2-treatment (E2) groups. The absolute VTG gene copy number per 1 µL cDNA in the liver is shown as mean ± SE (n = 5 individuals). (B). Absolute mRNA quantification of the four ER isoforms in liver, head kidney, immune cells isolated from head kidney and blood of 6-month-old rainbow trout treated with E2. The gene copy number of each isoform per 1 µL cDNA is presented by Box-Whisker plots (n = 5 individuals). Control and E2-treated group were compared for statistical analysis. The asterisks denote statistically significant differences between control and E2-treated groups. * p < 0.05, ** p < 0.01. a: under detection limit. b: Part of the sample was not detectable or under detection limit. c: not detected.