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. 2018 Apr 13;19(4):1195. doi: 10.3390/ijms19041195

Figure 1.

Figure 1

(a) Macroscopic and microscopic view of Alizarin red S (ALZR) staining of human primary osteoblasts (OB) co-cultured with 6, 9 or 12 alginate beads of human primary nucleus pulposus cells (NPC), annulus fibrosus cells (AFC), cartilaginous endplate cells (CEPC) or 6 empty beads (positive control) and negative control. All experimental groups were stimulated with osteogenic medium except the negative control, where OB were cultured with basal medium and (b) quantification of staining. Data is presented as min to max with median and all data points. Kruskal-Wallis test, * p-value < 0.05 N = 7. (c) Alkaline phosphatase (ALP) activity normalized to protein content was measured at day 10 and 21. Data is presented as min to max with median and all data points N = 7.