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. 2018 Feb 26;5(5):1700860. doi: 10.1002/advs.201700860

Figure 3.

Figure 3

Optimizing MSC–nanodrug conjugation. a) Phenotypic expression of the plasma membrane marker E‐cadherin on the surface of MSCs imaged by confocal microscopy confirmed that CD73 conjugation induced cell membrane damage after 48 h. b) Impact of CD conjugation on MSC migration. A comparison in migration between conjugated MSCs (i.e., CD90 and CD90 + CD73) and intracellularly loaded MSCs toward normal and cancer cell lines. Data represent mean ± SEM (n = 10), and the p value reference was MSCs. c) Amount of DOX conjugated with MSCconjugate and MSCupload. d) Percentage of MSC possessing DOX (for MSCconjugate and MSCupload). e) Migration analysis of MSCconjugate and MSCupload to A549 and H1975 cells. Data represent mean ± SEM (n = 10). f) FACS analysis of the apoptosis of MSC, MSCupload, and MSCconjugate after 24 h of nanodrug treatment (for reference, 1 × 104 single cell events were acquired).