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. 2018 Feb 26;5(5):1700860. doi: 10.1002/advs.201700860

Figure 4.

Figure 4

FACS analysis of H1975 and MSC apoptosis on co‐culture conditions. a) Time‐dependent FACS analysis of H1975 apoptosis when co‐cultured with MSCs, MSCupload, and MSCconjugate. Apoptotic H1975 cells were identified by BV421‐labeled annexin V. During the initial 72 h, apoptosis of lung cancer cells co‐cultured with MSCs steadily increased (i.e., MSCconjugate, 30.7%; MSCupload, 21.6%; and MSCs, 14.7%). However, after 240 h, lung cancer apoptosis decreased to negligible levels for MSCupload (4.3%) and MSCs (0.9%), whereas a continued increased in lung cancer apoptosis was observed with MSCconjugate (46.3%). b) Time‐dependent FACS analysis of MSC apoptosis when co‐cultured with H1975 cells was identified by BV421‐labeled annexin V.