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. 2018 Mar 22;42(1):61–70. doi: 10.3892/ijmm.2018.3587

Figure 5.

Figure 5

GlcN increases the expression of Wnt-4, Frizzled-2 and β-catenin, and decreases the expression of GSK-3β. (A) mRNA expression levels of Wnt-4, Frizzled-2, β-catenin and GSK-3β were determined by reverse transcription-polymerase chain reaction. (B) Western blot analysis was used to detect the protein expression levels of Wnt-4, Frizzled-2, β-catenin and GSK-3β. β-actin was used as an internal control. (C–F) Semi-quantification of Wnt-4, Frizzled-2, β-catenin and GSK-3β mRNA expression. (G-J) Semi-quantification of Wnt-4, Frizzled-2, β-catenin and GSK-3β protein expression. *P<0.05, **P<0.01 compared with untreated cells. GlcN, glucosamine; GSK-3β, glycogen synthase kinase-3β.