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Effect of Twist1 inhibition on expression of endothelial and smooth muscle-like cell markers in pulmonary microvascular endothelial cells. Western blot analysis demonstrated that siTwist1 obviously increased CD31 expression and decreased α-SMA and Col1A1 expression upon hypoxic exposure. *P<0.05 vs. control; #P<0.05 vs. hypoxia group. SMA, smooth muscle actin; Col, collagen; siTwist1, small interfering RNA targeting Twist1.
