Figure 5.

PFK15 regulates AMPK proteins expression. (A) RD cells were treated for 2, 4 and 12 h with 6 μM PFK15, and western blot analysis was conducted to examine the levels of t-AMPK, p-AMPK and p-ACC proteins. Total ERK was used as a loading control. (B) Immunoblotting assay was performed to determine the p-mTOR, p-AKT and t-AKT levels in cells treated with PFK15 (6 μM). RD, rhabdomyosarcoma; AMPK, adenosine monophosphate-activated protein kinase; AKT, protein kinase B; ACC, acetyl-CoA carboxylase; ERK, extracellular signal-regulated kinase; mTOR, mammalian target of rapamycin; p-, phosphorylated; t-, total; Ctrl, control.