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Altered expression of pluripotency-associated genes confirmed by reverse transcription-quantitative polymerase chain reaction analysis. The results showed the significant upregulation of Tbx-3 and PI3K(85α) (P<0.01). The mRNA level of the control group cultured in the absence of LIF was set as 1. *P<0.05 and ***P<0.001 (n=3). LIF, leukemia inhibitory factor; bFGF, basic fibroblast growth factor; Tbx-3, T-box 3; PI3K, phosphoinositide 3-kinase; Oct-4, octamer-binding protein 4.
