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. 2018 Mar 20;42(1):248–258. doi: 10.3892/ijmm.2018.3577

Figure 2.

Figure 2

Nontypeable Haemophilus influenzae (NTHi)-stimulated chemokine CXC receptor 4 (CXCR4) activation relies on TLR3/MyD88 signaling pathway. human middle ear epithelial cells (HMEECs) were transfected with the control, (A) TLR3-, TLR4-, (B) MyD88-, IRAK1-, (C) TRAF6- and TAK1-mutant plasmids. Then, the cells were exposed to NTHi for 6 h. The CXCR4 mRNA levels were then measured through RT-qPCR analysis. HMEECs were transfected with the Control siRNA, (D) TLR3-, TLR4-, (E) MyD88-, IRAK1-, (F) TRAF6- and TAK1-siRNA for knockdown. CXCR4 gene levels in cells were evaluated via RT-qPCR analysis. Representative data are shown as SEM. *p<0.05, **p<0.01 and ***p<0.001 vs. the group in the absence of gene mutants or silence.