Figure 5.

Juglanin induces caspase activation and PARP-1 cleavage. (A) Immunofluorescence analysis of caspase-3 was conducted on tissue sections. Representative photomicrographs from each group were shown (scale bar, 100 μm). (B) Western blotting was conducted and relative protein expression levels of C-caspase-3, C-caspase-8 and C-PARP were determined. Equal protein loading was confirmed by stripping the immunoblot and reprobing it for GAPDH. Data are presented as the means ± standard deviation of three independent experiments. ^##P<0.01 and ^###P<0.001 compared with the Con group; ^*P<0.05, ^**P<0.01 and ^***P<0.001 compared with the Mod group. C-, cleaved; Con, control; JugH, high juglanin; JugL, low juglanin; Mod, model; PARP, poly (ADP-ribose) polymerase; UVB, ultraviolet B.