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. 2018 Mar 22;42(1):237–247. doi: 10.3892/ijmm.2018.3585

Figure 3.

Figure 3

Fisetin ameliorates lipopolysaccharide (LPS)-induced inflammation in the middle ear of mice through Toll-like receptor 4 (TLR4)/nuclear factor-κB (NF-κB) signaling pathway. Western blot analysis was conducted to explore (A) TLR4 and (B) MyD88 protein expression levels. (C) TLR4 and (D) MyD88 mRNA levels were measured via RT-qPCR assays, and the relative fold quantification is exhibited. The immunoblotting analysis was performed to determine (E) p-IKKα, (F) p-IκBα and (G) p-NF-κB protein levels in the middle ear of mice. Data are expressed as the mean ± SEM (n=10). *p<0.05, **p<0.01 and ***p<0.001 vs. the control (Con) group; +p<0.05, ++p<0.01 and +++p<0.001 vs. the LPS group.