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. 2018 Mar 30;42(1):346–358. doi: 10.3892/ijmm.2018.3606

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Copyright: © Wu et al.

This is an open access article distributed under the terms of the Creative Commons Attribution-NonCommercial-NoDerivs License, which permits use and distribution in any medium, provided the original work is properly cited, the use is non-commercial and no modifications or adaptations are made.

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Orthotopic liver transplantation increases KC activation and TIM-4 expression. (A) KCs were isolated from mice in the sham and LT groups 24, 48 and 72 h following surgery. Cells were double stained with PE-CD14 and APC-CD163 and quantified using flow cytometry. (B) Liver samples were obtained from mice in the sham and LT groups and assessed using immunohistochemistry (magnification, ×400). Activated hepatic KCs are stained brown. (C) KCs were isolated from mice in the sham and LT groups 1, 3 and 7 days following surgery and TIM-4 expression was analyzed using western blotting. Data are presented as the mean ± standard deviation. ^aP<0.05 vs. sham group. KC, kupffer cells; TIM-4, T cell immunoglobulin-domain mucin-domain-4; CD, cluster of differentiation; APC, antigen presenting cell; LT, liver transplantation; PE, phycoerythrin; d, days.

Orthotopic liver transplantation increases KC activation and TIM-4 expression. (A) KCs were isolated from mice in the sham and LT groups 24, 48 and 72 h following surgery. Cells were double stained with PE-CD14 and APC-CD163 and quantified using flow cytometry. (B) Liver samples were obtained from mice in the sham and LT groups and assessed using immunohistochemistry (magnification, ×400). Activated hepatic KCs are stained brown. (C) KCs were isolated from mice in the sham and LT groups 1, 3 and 7 days following surgery and TIM-4 expression was analyzed using western blotting. Data are presented as the mean ± standard deviation. ^aP<0.05 vs. sham group. KC, kupffer cells; TIM-4, T cell immunoglobulin-domain mucin-domain-4; CD, cluster of differentiation; APC, antigen presenting cell; LT, liver transplantation; PE, phycoerythrin; d, days.